HPLC Column
High Performance Liquid Chromatography (HPLC)
This technique is a form of column chromatography used frequently in biochemistry and analytical chemistry. It involves passing a mixture containing the “analyte” through a column (stationary phase), by a liquid (mobile phase) at high pressure.
Cation exchange chromatography is a process that allows the separation of the mixture based on the charge properties of the molecules in the mixture. Cation exchange chromatography retains analyte molecules based on coulombic (ionic) interactions.
The stationary phase surface displays negatively charged functional groups that interact with positively charged cations in the mixture.